Characterization of Stem Bark of Mangifera Indica and Its Tanning Potentials

J. U. Akajagbor, P. O. Ukoha, C. C. Maju, I. Akawu

Abstract


The stem bark of mangifera indica was dried, ground and extracted with methanol at 32 oc.   The extract was fractionated using hexane, ethyl acetate and acetone, which were characterized via UV, IR, H-NMR & C-NMR spectroscopy.  The solid methanolic extract was analyzed for its tannin content based on tannic acid and folinciocalted methods, which gave 34.84±2.2 and 32.33 ± 1.4 respectively.  The two way ANOVA of results for these two methods shows that the mean difference for the two methods is not significant.  The chromatographic separation gave RF values of 0.12 and 0.13 using 6% acetic acid as mobile phase gave RF values of 0.88, and 0.36, thus implicating methyl –M- digallate, mangiferin, B – Penta –O- galloy –D- glucose and Chromatographic separation gave Rf values of 0.12 and 0.13 using 6% acetic acid as mobile phase, while n-butano1-acetic/H2O (14:1:5) as mobile phase gave Rf values of 0.88 and 0.36, Thus implicating methy-M-digallate, mangiferin, B-penta-o-galloy-D-Glucose and 1.6 digalloy1-D-glucose as components of mangifera indica.  IR analysis conducted on the fractions of the plant materials shows the following bands (cm-1) at 3382.29,2929.97, 1629.90, 1493.92 and 1261.49.  Proton NMR shows signals (ppm) at 7.68 – 6.19, 5.20 while carbon-NMR shows signals (ppm) at 176.36, 167.81 – 93.91.  The leather produced from stem bark of mangifera indica was supple, soft, exhibit good compressibility and water-resistant characteristics.  The physicochemical carried out for the leather was favourable for the production of leather products such as car upholstery, football, belt, shoe upper and insole.


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References


Kantie, E., Richard, W. (2006). Squirrels; the animal answer guide. Baltimore: Johns Hopkins University Press P. 91 ISBN )-8018-8402

Li, Jingge, Maples den, Frances (1998). “ Commercial Production of tannins from radiate’ pine bark of wood adhesives†PDF/PENZ Transaction 25 (1/Emch)

Hemingway, R.W and Karchessy, J.J. (Ed) 1992. Chemistry and significance of condensed tannins, plenum Press. NYPP. 266-267

Torres, J., Olivares, S., De la Rosa, D., Lima, L, Martinez, F., Munita, C.S., Favaro, D I.T (1999) “Removal of Mecury II and methyl mercury from a solution of tannin absorbents. Journal of Radioanalytical and Nuclear Chemistry 240(1): 361 -5 doi 1007/BF 02349180.

Takashi Sakaguchai; Akira Nakajonaa (1987). “Recovery of uranium from seawater by immobilized tanninâ€. Separation Science and Technology 22(6) 1609-23 doi 10 1080/0149698-708058421

Kakhonem, M.P., Hopia, A.I., Vourela, H.J., Raula, J., Philaja, K, Kujala, T.S., and Heinomen, M., (1999), Anti-oxidant activity of plant extract containing phenolic compounds, J. Agric. Food Chem. 47,3954-3962.

Rice – Evans, C.A., Miller – Paganga, J. (1997). Anti – oxidant properties of phenolic compounds, Trends plant Sci., 2,152 – 159

Chem, X.Y., Ahn, D.U., (1973). Anti – oxidant properties of six natural phenolics against lipid oxidation induced by Fe2+ or ultraviolet light J. Am Oil Chem. Soc 73,1717 – 1721

Luiz, M., Biasuth, A., and Garcia, N.A., and Micellar, F., (2000). Effect of scavenging of singlet molecular oxygen by Hydroxybenzene Redox Rep. 7,23 – 28

Ihuoma, A. A (1993): Nigeria’s Leather Trades and Economy: 1960 – 1987. Journal of Leather & Chemical Technology Vol 7, No1, 1993

Ukoha, P.O. Ejila A., Maju, C., and Asegbeloyin, J.N. (2008). Tannic Acid as a standard for the spectrophotometric determination of tannins; J. Chem. Soc. Nigeria, 33, 181 – 186.


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