In Vitro Antioxidant Activity of Stem Bark Extracts of Albizia Ferrugenea Fabaceae (Guill & Perr)

Aisha Y. Lawal, Babale Abdullahi, Isah Abdulazeez

Abstract


Antioxidant activity of methanol and ethyl acetate extract of Albizia ferruginea (Guill. & Perr.)Stembark used in traditional medicine was determined using the free radical scavenging activity of the 1, 1-diphenyl-2-picrylhydrazyl radical (DPPH), total phenolics content and reducing power assay on the extract was also determined.  The results of the DPPH scavenging activity study indicate a concentration dependent antioxidant activity with 46.90, 56.32, 68.74, 77.24 and 84.60% for methanol extract and 51.26, 68.74, 70.12, 88.55 and 90.05 for ethyl acetate extract of various concentrations of 10, 25, 50,125 and 250 μg/ml respectively. The total phenolic content was found to be 54.37 mg/g Gallic acid equivalent (GAE). The DPPH scavenging activity of the extract was found to be promising. The extracts showed significant antioxidant activity compared with the standard Ascorbic and Gallic acids. The phytochemical screening revealed the presence of flavonoids, tannins, saponins and steroids. It indicates that the extracts of the plant have the potency of  scavenging free radicals in vitro and may provide leads in the ongoing search for natural antioxidants from medicinal plants to be used in treating diseases related to free radical reactions.


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