Isolation and Characterization of Microorganisms Consortia Associated with Biogas Production from Spinach and Selected Fruits Waste as Substrates

Tambuwal A. D., Ogbiko C.

Abstract


The research study was aimed at investigating microorganisms associated with biogas production using vegetable (Spinacia oleracea), banana peel, plantain peel, watermelon residue and pumpkin wastes as substrates. The samples were procured from different locations within Sokoto metropolis. The study was completed within a period of five weeks (35 days). Standard microbiological methods and fabricated anaerobic bio-digesters were used to screen the isolates and the wastes substrate for biogas production. Analysis revealed that the temperature within the digester ranged between 30°C and 34°C while the pH varied between 6.30 and 7.20 and 5.0 – 6.2 before and during/after the anaerobic digestion respectively. Anaerobic bacteria isolated were identified as Staphylococcus aureus, Micrococcus spp, Enterobacter spp, Escherichia coli, Citrobacter diversus, Bacillus firmus and Pseudomonas aeruginosa. The result showed that the percentage yield of biogas produced from the substrate is in the order: synergized mixture > plantain > banana > pumpkin > spinach > watermelon. The volume of biogas produced varied significantly (p<0.05) between the substrate treatments and the digestion internals (days). This study has confirmed the role of methanogens and other complementing bacteria in biogas production.


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Adelekan, B. and Bamgboye, A. (2009). Comparison of biogas productivity of cassava peels mixed in selected ratios with major livestock waste types. African Journal of Agricultural Research. 4: 571-577

Aderonke, A., Wasiu, A. and Moses, O. (2017). Isolation and Characterization of Microorganisms with Hydrolytic Profile during Anaerobic Digestion and Biogas Production of Cow Dung and Rice Husk. Journal of Natural Sciences Research. 7(5): 20-30

Angelidaki, I., Ellegaard, L. and Ahring, B.K.A. (1993). Mathematical model for dynamic simulation of anaerobic digestion of complex substrates: focusing on ammonia inhibition. Biotechnology and Bioengineering, 42: 159–166.

Anuputtikul, W. and Rodtong, S. (2004). Investigation of the potential production of biogas from cassava tuber. 70: 12-23.

Aremu, M. and Agarry, S. (2012). Comparison of biogas production from cow dung and pig dung under mesophilic condition. International Referred Journal of Engineering and Science. 4:16-21.

Asikong, B.E., Idire, S.O. and Tiku, D.R. (2016). Microorganisms Associated with Biogas Production Using Vegetable (Telfairia occidentalis) Wastes, Banana Peel and Pig Dung as Substrates, British Microbiology Research Journal. 16(3): 1-12.

Baba, S., Shedu, U., Abubakar, I. and Nasir, I. (2012). Anaerobic digestion of cow dung for biogas production. ARPN Journal of Engineering and Applied Sciences.7:169-172.

Bagudo, B.U. (2007). “Studies on Biogas Production from Selected Agricultural Waste Materials†A Ph.D Thesis submitted to Postgraduate School Usmanu Danfodiyo University Sokoto.

Bitton, G. (2005). Wastewater Microbiology (A John Wiley & Sons Inc. Publication, 3rd ed. Hoboken, New Jersey Chap. 13: 345–369.

Demirel, B. and Scherer, P. (2008). The roles of acetotrophic and hydrogenotrophic methanogens during anaerobic conversion of biomass of methane. Review of Environmental Science and Technology. 7: 73-901.

Dhevagi, P., Ramasamy, R. and Oblisami, G. (1992). Biological nitrogen fixation and biogas technology. Bioresource Technology. 6: 14-23.

El-Mashed, H., Zeeman, G., Loon, W., Bot, G. and Lettinga G. (2003). Effect of temperature fluctuation on thermopholic anaerobic digestion of cattle manure. Bioresources Technology. 95:213-221.

Ezeohu, S., Dioha, I. and Eboatu, N. (2005). Daily biogas production from different wastes and identification of methanogenic bacteria involved. Nigerian Journal of Solar Energy. 15: 80-85.

Garba, B. and. Sambo, A.S. (1992). “Effect of Operating Parameters on Biogas Production Rateâ€.Nigerian Journal of Renewable Energy. 3(1 and 2): 36 - 44.

Gerardi, M.H. (2003). Wastewater microbiology series: The microbiology of anaerobic digesters. John Wiley and Sons Inc., New York.

Gopinath, L.R., Merlin C.P., Mahesh, K., Bhuvaneswari, R. and Divya, D. (2014). Identification and Evaluation of Effective Bacterial Consortia for Efficient Biogas Production. IOSR Journal of Environmental Science, Toxicology and Food Technology. 8(3): 80-86

Gregersen, T. (1978). Rapid method for distinction of gram-negative from gram-positive bacteria. Appl. Micobiol. Biotechnol. 5(2): 123-127.

Holt, J.G., Krieg, N.R., Sneath, P.H., Staley, J.T., Williams, S.T. (1994). Bergey’s Manual of Determinative Bacteriology, 9th Edition. (H. W. R, Ed.). Williams &Wilkins.

Jaenicke, S., Zakzewski, M., Ander, C. and Bekel, T. (2011). Comparative and joint analysis of two metagenomic data sets from a biogas fermenter by 454-phyro sequencing. Plus One. 6(1): 14-19.

Jianzheng, L., Ajay, K., Junguo, H., Qiaoying, B., Sheng, C. and Peng, W. (2011). Assessment of the effects of dry anaerobic co-digestion of cow dung with waste water sludge on biogas yield and biodegradability. Int. J. Phys. Sci. 6(15): 3679-3688.

Li, X., Li, L., Zheng, M., Fu, G. and Lar, J. (2009). Anaerobic co-digestion of cattle manure with corn Stover pretreated by sodium hydroxide for efficient biogas production. Energ. Fuel. 23: 4635-4639.

Madu, C. and Sodeinde, O. (2001). Relevance of biomass in the sustainable energy development in Nigeria. Proceedings of the national engineering conference and annual general meeting of the Nigerian Society of Engineers. pp. 220-227

Mata-Alvarez, J., Mace, S. and Labres, P. (2000). Anaerobic digestion of organic solid wastes. An overview of research achievements and perspectives. Rev. Paper Bio-resource Technol. 74:3-16.

Nwuche, C. O. and Ugoji E. O. (2008). Effects of heavy metal pollution on the soil microbial activity. International Journal of Environmental Science and Technology, 5(3): 409-414.

Nwuche, C. O. and Ugoji E. O. (2010). Effect of co-existing plant species on soil microbial activity under heavy metal stress. International Journal of Environmental Science and Technology. 7(4): 697-704.

Roland, W., Etelka, K., Gergely, M., Zoltan, B., Gabor, R. and Kornel, L.K. (2012). Characterization of a biogas-producing microbial community by short-read next generation DNA sequencing. Biotechnology for Biofuels. 5(41): 231 – 241.

Schink, B. (1997). Energetics of syntrophic cooperation in methanogenic degradation. Microbiology and Molecular Biology Reviews. 61(2): 262–280.

Surnaso, S., Siswo, S. and Budiyono, Y. (2010). Biogas production using anaerobic biodigester from cassava starch effluent. International Journal of Science and Engineering. 1(2):33-37.

Tsunatu, D., Yavini, U., Usman, H., Taura, N. and James, M. (2014). Comparative study of mesophilic biogas production potentials of selected agro-wastes. International Journal of Engineering and Science. 3(2):1-6.

Yerima, M.B, Rahman A.T.M.F. and Ekwenchi, M.M. (2001).â€Effect of Buffering on Biogas Fermentation of Chicken Droppings†Nigerian Journal of Renewable Energy, 9(1 and 2): 47-49

Ziemiński, K. and Magdalena, F. (2012). Methane fermentation process as anaerobic digestion of biomass: Transformations, stages and microorganisms. African Journal of Biotechnology. 11(18): 4127-4139.


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